Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer

Şahingöz, İskender; IMAMOGLU, RIZVAN; GOKCE, Isa; Kaplan, Ozlem

doi:10.35860/iarej.766741

Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer

Özlem KAPLAN, (İstanbul Üniversitesi, Moleküler Biyoloji ve Genetik Bölümü, İstanbul, Türkiye)

Rizvan İMAMOĞLU, (Bartın Üniversitesi, Biyoteknoloji Bölümü, Bartın, Türkiye)

İskender ŞAHİNGÖZ, (Tokat Gaziosmanpaşa Üniversitesi, Genetik ve Biyomühendislik Bölümü, Tokat, Türkiye)

İsa GÖKÇE (Tokat Gaziosmanpaşa Üniversitesi, Genetik ve Biyomühendislik Bölümü, Tokat, Türkiye)

International Advanced Researches and Engineering Journal

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Yıl: 2021 Cilt: 5 Sayı: 1 Sayfa Aralığı: 42 - 46 Metin Dili: İngilizce DOI: 10.35860/iarej.766741 İndeks Tarihi: 29-07-2022

Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer

Öz:

Single-stranded DNA-binding (SSB) proteins play an important role in DNA metabolism involving DNA replication, recombination, and repair in all living beings. In molecular biology, SSB proteins are used as enhancers to increase the efficiency and specificity of PCR. Thermostable SSB protein eliminates secondary structure or dimer formation and significantly increase the effectiveness of amplification of DNA fragments. In this study, it was ensured that the SSB gene of thermophilic bacteria Thermus aquaticus (T. aquaticus) was cloned into the pET28b vector and expressed in E. coli BL21 (DE3) PLysE cells. Then, the purification of the SSB protein produced in E. coli BL21 (DE3) PLysE cells was performed. 20 mg SSB protein was obtained from 1L bacterial culture, and its purity was more than 90%. It was shown by the PCR experiment that the SSB protein produced in this study could increase the amplification efficiency.

Anahtar Kelime: Single Strand Binding Protein Thermus aquaticus Polymerase Chain Reaction

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Kaplan O, IMAMOGLU R, Şahingöz İ, GOKCE I (2021). Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer. , 42 - 46. 10.35860/iarej.766741
Chicago	Kaplan Ozlem,IMAMOGLU RIZVAN,Şahingöz İskender,GOKCE Isa Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer. (2021): 42 - 46. 10.35860/iarej.766741
MLA	Kaplan Ozlem,IMAMOGLU RIZVAN,Şahingöz İskender,GOKCE Isa Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer. , 2021, ss.42 - 46. 10.35860/iarej.766741
AMA	Kaplan O,IMAMOGLU R,Şahingöz İ,GOKCE I Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer. . 2021; 42 - 46. 10.35860/iarej.766741
Vancouver	Kaplan O,IMAMOGLU R,Şahingöz İ,GOKCE I Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer. . 2021; 42 - 46. 10.35860/iarej.766741
IEEE	Kaplan O,IMAMOGLU R,Şahingöz İ,GOKCE I "Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer." , ss.42 - 46, 2021. 10.35860/iarej.766741
ISNAD	Kaplan, Ozlem vd. "Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer". (2021), 42-46. https://doi.org/10.35860/iarej.766741

APA	Kaplan O, IMAMOGLU R, Şahingöz İ, GOKCE I (2021). Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer. International Advanced Researches and Engineering Journal, 5(1), 42 - 46. 10.35860/iarej.766741
Chicago	Kaplan Ozlem,IMAMOGLU RIZVAN,Şahingöz İskender,GOKCE Isa Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer. International Advanced Researches and Engineering Journal 5, no.1 (2021): 42 - 46. 10.35860/iarej.766741
MLA	Kaplan Ozlem,IMAMOGLU RIZVAN,Şahingöz İskender,GOKCE Isa Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer. International Advanced Researches and Engineering Journal, vol.5, no.1, 2021, ss.42 - 46. 10.35860/iarej.766741
AMA	Kaplan O,IMAMOGLU R,Şahingöz İ,GOKCE I Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer. International Advanced Researches and Engineering Journal. 2021; 5(1): 42 - 46. 10.35860/iarej.766741
Vancouver	Kaplan O,IMAMOGLU R,Şahingöz İ,GOKCE I Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer. International Advanced Researches and Engineering Journal. 2021; 5(1): 42 - 46. 10.35860/iarej.766741
IEEE	Kaplan O,IMAMOGLU R,Şahingöz İ,GOKCE I "Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer." International Advanced Researches and Engineering Journal, 5, ss.42 - 46, 2021. 10.35860/iarej.766741
ISNAD	Kaplan, Ozlem vd. "Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer". International Advanced Researches and Engineering Journal 5/1 (2021), 42-46. https://doi.org/10.35860/iarej.766741