A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model

yılmaz, sarper; KURT OZKAYA, Nese; ZEREYAK, UMUT; İNAN, Zeynep Deniz Şahin

doi:10.5455/annalsmedres.2020.08.879

A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model

Neşe KURT ÖZKAYA, (Sivas Cumhuriyet Üniversitesi, Tıp Fakültesi, Plastik Rekonstrüktif Estetik Cerrahi Anabilim Dalı, Sivas, Türkiye)

Zeynep Deniz ŞAHİN İNAN, (Sivas Cumhuriyet Üniversitesi, Tıp Fakültesi, Histoloji-Embriyoloji Anabilim Dalı, Sivas, Türkiye)

Umut ZEREYAK, (Özel Medicana Çamlıca Hastanesi, Plastik Rekonstrüktif Estetik Cerrahi Anabilim Dalı, İstanbul, Türkiye)

Sarper YILMAZ (Özel Güven Hastanesi, Plastik Rekonstrüktif Estetik Cerrahi Anabilim Dalı, Ankara, Türkiye)

Annals of Medical Research

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Yıl: 2021 Cilt: 28 Sayı: 7 Sayfa Aralığı: 1387 - 1394 Metin Dili: İngilizce DOI: 10.5455/annalsmedres.2020.08.879 İndeks Tarihi: 07-11-2021

A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model

Öz:

Aim: Platelet Rich Plasma (PRP) is a method used in dentistry and aesthetic applications in order to accelerate tissue healing. The purpose of this study is to assess the effect of PRP on accelerating wound healing and the contribution of microneedling application, i.e. multiple injection during PRP, and the volume effect of the PRP material to healing.Materials and Methods: In the study, 16 5-month-old male white New Zealand rabbits with an average weight of 2.7-3.0 kg were used. The four wounds were made on the back of each rabbit, and PRP, Normal saline(NS), microneedling(MN), and Sham procedures applied. Eight rabbits were sacrificed by taking their tissues for histological assessment at the end of 14 days. Epithelization, VEGF expression, angiogenesis, inflammatory cell, fibroblast, reticular fiber, and collagen parameters were evaluated. Eight rabbits were followed up for macroscopic healing. Result: The PRP procedure decreased the healing duration and wound area compared to other procedures(p<0.05). The NS procedure decreased the healing duration compared to the Sham procedure and provided a significant decrease in wound area compared to the MN and Sham procedures(p<0.05). Although the MN procedure decreased the wound area compared the Sham procedure, this difference was not statistically significant(p>0.05). PRP procedure increased staining scores and densities of epithelization, VEGF expression, angiogenesis compared to Sham procedure(p<0.05). PRP procedure increased staining score of collagen compared to Sham and NS procedures and staining density of collagen compared to other procedures(p<0.05). MN procedure decreased staining density of inflammatory cell compared to Sham procedure(p<0.05). Conclusion: The evaluation of simultaneous microneedling and PRP procedure on the 14th day of wound healing did not provide more positive synergistic effects on healing. In order to reduce the volume effect of the PRP amount given, the amount of PRP applied at each point should be as low as possible.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık

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APA	KURT OZKAYA N, İNAN Z, ZEREYAK U, yılmaz s (2021). A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model. , 1387 - 1394. 10.5455/annalsmedres.2020.08.879
Chicago	KURT OZKAYA Nese,İNAN Zeynep Deniz Şahin,ZEREYAK UMUT,yılmaz sarper A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model. (2021): 1387 - 1394. 10.5455/annalsmedres.2020.08.879
MLA	KURT OZKAYA Nese,İNAN Zeynep Deniz Şahin,ZEREYAK UMUT,yılmaz sarper A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model. , 2021, ss.1387 - 1394. 10.5455/annalsmedres.2020.08.879
AMA	KURT OZKAYA N,İNAN Z,ZEREYAK U,yılmaz s A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model. . 2021; 1387 - 1394. 10.5455/annalsmedres.2020.08.879
Vancouver	KURT OZKAYA N,İNAN Z,ZEREYAK U,yılmaz s A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model. . 2021; 1387 - 1394. 10.5455/annalsmedres.2020.08.879
IEEE	KURT OZKAYA N,İNAN Z,ZEREYAK U,yılmaz s "A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model." , ss.1387 - 1394, 2021. 10.5455/annalsmedres.2020.08.879
ISNAD	KURT OZKAYA, Nese vd. "A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model". (2021), 1387-1394. https://doi.org/10.5455/annalsmedres.2020.08.879

APA	KURT OZKAYA N, İNAN Z, ZEREYAK U, yılmaz s (2021). A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model. Annals of Medical Research, 28(7), 1387 - 1394. 10.5455/annalsmedres.2020.08.879
Chicago	KURT OZKAYA Nese,İNAN Zeynep Deniz Şahin,ZEREYAK UMUT,yılmaz sarper A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model. Annals of Medical Research 28, no.7 (2021): 1387 - 1394. 10.5455/annalsmedres.2020.08.879
MLA	KURT OZKAYA Nese,İNAN Zeynep Deniz Şahin,ZEREYAK UMUT,yılmaz sarper A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model. Annals of Medical Research, vol.28, no.7, 2021, ss.1387 - 1394. 10.5455/annalsmedres.2020.08.879
AMA	KURT OZKAYA N,İNAN Z,ZEREYAK U,yılmaz s A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model. Annals of Medical Research. 2021; 28(7): 1387 - 1394. 10.5455/annalsmedres.2020.08.879
Vancouver	KURT OZKAYA N,İNAN Z,ZEREYAK U,yılmaz s A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model. Annals of Medical Research. 2021; 28(7): 1387 - 1394. 10.5455/annalsmedres.2020.08.879
IEEE	KURT OZKAYA N,İNAN Z,ZEREYAK U,yılmaz s "A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model." Annals of Medical Research, 28, ss.1387 - 1394, 2021. 10.5455/annalsmedres.2020.08.879
ISNAD	KURT OZKAYA, Nese vd. "A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model". Annals of Medical Research 28/7 (2021), 1387-1394. https://doi.org/10.5455/annalsmedres.2020.08.879