In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells
Yıl: 2021 Cilt: 18 Sayı: 5 Sayfa Aralığı: 87 - 90 Metin Dili: İngilizce DOI: 10.4274/tjps.galenos.2021.04903 İndeks Tarihi: 19-01-2022
In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells
Öz: Objectives: Traditional treatment methods are becoming popular and commonly used in many societies and have become the first treatment option for most people. While some of these methods are helpful, they can interact with medications the patient is taking for another disease and cause a variety of life-threatening risks. Valerian (catweed) plant is used in traditional medicine as a sleep aid due to its sedative effects. Valerian may also exert anticancer effect in vitro. Materials and Methods: In this study, the cytotoxicty and oxidative stress effects of valerian root extract were evaluated in human liver hepatocellular carcinoma (Hepg2) and human colorectal adenocarcinoma (Caco2) cell lines. The cytotoxicity was evaluated via the 3-(4,5-dimethythiazol-2-yl)- 2,5-diphenyl tetrazolium bromide test. Total reactive oxygen species analysis was performed via a 2’,7’-dichlorodihydrofluorescein diacetate assay in flow cytometry. Results: Inhibition concentration 50 values were calculated as 936.6 and 1097.5 µg/mL in the Hepg2 and Caco2 cell lines, respectively. It was observed that valerian root extract did not induce oxidative stress in HepG2 and Caco2 cell lines. Conclusion: These results indicate that the use of valerian root extract as an alternative method in cancer treatment may not be effective and may cause a risk for public health. On the other hand, it may be safe at recommended tolerated concentrations since it does not cause oxidative stress.
Anahtar Kelime: Valeriana officinalis’in Metanol ile Hazırlanmış Ekstresinin HepG2 ve Caco2 Hücrelerinde İn Vitro Sitotoksisite ve Oksidatif Stres Değerlendirmesi
Öz: Amaç: Geleneksel tedavi yöntemleri, birçok toplumda yaygın olarak kullanılmakta, popüler hale gelmekte ve birçok kişi için ilk tedavi seçeneği olarak karşımıza çıkmaktadır. Bu yöntemlerden bazıları yararlı olmakla birlikte, kişinin başka bir hastalık için kullandığı ilaçlarla etkileşime girebilir ve çeşitli yaşamı tehdit edici risklere neden olabilir. Kediotu (catweed) bitkisi yatıştırıcı etkisi nedeniyle geleneksel tıp uygulamalarında uyku düzenleyici amaçlı kullanılmaktadır. Ayrıca in vitro olarak kanser önleyici etkiye sahip olabileceği bildirilmiştir. Gereç ve Yöntemler: Bu çalışmada, kediotu kökü ekstresinin sitotoksisite ve oksidatif stres etkileri Hepg2 ve Caco2 hücre hatlarında değerlendirilmiştir. Sitotoksisite değerlendirmesi 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide testi ile gerçekleştirildi. Total-reaktif oksijen bileşikleri analizi, hücre akış sitometrisinde 2’,7’-dichlorodihydrofluorescein diacetate testi ile gerçekleştirildi Bulgular: İnhibisyon konsantrasyonu 50 değerleri Hepg2’de 936,6 µg/mL ve Caco2 hücre hatlarında 1097,5 µg/mL olarak hesaplandı. Kediotu kökü ekstresinin HepG2 ve Caco2 hücre hatlarında da oksidatif strese neden olmadığı gözlenmiştir. Sonuç: Bu sonuçlar, kanser tedavisinde alternatif bir yöntem olarak kediotu kökü ekstresi kullanımının etkili olamayacağını ve halk sağlığı açısından risk oluşturabileceğini, diğer yandan oksidatif strese neden olmadığı için tavsiye edilen tolere edilen konsantrasyonlarda güvenli olabileceğini göstermektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Kara M, Alparslan E, Öztaş E, ERDOĞAN Ö (2021). In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells. , 87 - 90. 10.4274/tjps.galenos.2021.04903 |
| Chicago | Kara Mehtap,Alparslan Ecem Dilara,Öztaş Ezgi,ERDOĞAN ÖZLEM NAZAN In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells. (2021): 87 - 90. 10.4274/tjps.galenos.2021.04903 |
| MLA | Kara Mehtap,Alparslan Ecem Dilara,Öztaş Ezgi,ERDOĞAN ÖZLEM NAZAN In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells. , 2021, ss.87 - 90. 10.4274/tjps.galenos.2021.04903 |
| AMA | Kara M,Alparslan E,Öztaş E,ERDOĞAN Ö In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells. . 2021; 87 - 90. 10.4274/tjps.galenos.2021.04903 |
| Vancouver | Kara M,Alparslan E,Öztaş E,ERDOĞAN Ö In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells. . 2021; 87 - 90. 10.4274/tjps.galenos.2021.04903 |
| IEEE | Kara M,Alparslan E,Öztaş E,ERDOĞAN Ö "In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells." , ss.87 - 90, 2021. 10.4274/tjps.galenos.2021.04903 |
| ISNAD | Kara, Mehtap vd. "In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells". (2021), 87-90. https://doi.org/10.4274/tjps.galenos.2021.04903 |
| APA | Kara M, Alparslan E, Öztaş E, ERDOĞAN Ö (2021). In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells. Turkish Journal of Pharmaceutical Sciences, 18(5), 87 - 90. 10.4274/tjps.galenos.2021.04903 |
| Chicago | Kara Mehtap,Alparslan Ecem Dilara,Öztaş Ezgi,ERDOĞAN ÖZLEM NAZAN In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells. Turkish Journal of Pharmaceutical Sciences 18, no.5 (2021): 87 - 90. 10.4274/tjps.galenos.2021.04903 |
| MLA | Kara Mehtap,Alparslan Ecem Dilara,Öztaş Ezgi,ERDOĞAN ÖZLEM NAZAN In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells. Turkish Journal of Pharmaceutical Sciences, vol.18, no.5, 2021, ss.87 - 90. 10.4274/tjps.galenos.2021.04903 |
| AMA | Kara M,Alparslan E,Öztaş E,ERDOĞAN Ö In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells. Turkish Journal of Pharmaceutical Sciences. 2021; 18(5): 87 - 90. 10.4274/tjps.galenos.2021.04903 |
| Vancouver | Kara M,Alparslan E,Öztaş E,ERDOĞAN Ö In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells. Turkish Journal of Pharmaceutical Sciences. 2021; 18(5): 87 - 90. 10.4274/tjps.galenos.2021.04903 |
| IEEE | Kara M,Alparslan E,Öztaş E,ERDOĞAN Ö "In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells." Turkish Journal of Pharmaceutical Sciences, 18, ss.87 - 90, 2021. 10.4274/tjps.galenos.2021.04903 |
| ISNAD | Kara, Mehtap vd. "In Vitro Cytotoxicity and Oxidative Stress Evaluation of Valerian (Valeriana officinalis) Methanolic Extract in Hepg2 and Caco2 Cells". Turkish Journal of Pharmaceutical Sciences 18/5 (2021), 87-90. https://doi.org/10.4274/tjps.galenos.2021.04903 |
