Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma
Yıl: 2021 Cilt: 51 Sayı: 2 Sayfa Aralığı: 638 - 643 Metin Dili: İngilizce DOI: 10.3906/sag-2004-214 İndeks Tarihi: 21-01-2022
Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma
Öz: Background/aim: Hepatocellular carcinoma (HCC) is one of the most aggressive cancer types. MicroRNAs (miRNAs) are small noncoding regulatory RNAs that function posttranscriptionally. miRNA deregulation was observed in the development and progression of HCC. In this study, we aimed to investigate the expression levels of four miRNAs (mir-33a, mir-203b, mir361-3p, and mir-424) in HCC patients in comparison to healthy individuals. Materials and methods: Venous blood samples were collected from both HCC patients and healthy individuals. In order to determine the relative expression levels of mir-33a, mir-203b, mir361-3p, and hsa-mir-424 in HCC patients, probe-based quantitative real time PCR (qRT-PCR) was performed. The cycle threshold (Ct) results were analyzed according to the 2−∆∆Ct method and statistical analyses were performed by SPSS Statistics version 15 for Windows. Results: qRT-PCR analysis revealed that the expression levels of mir-33a (fold change: 7.3 and P < 0.001), mir-203b (fold change: 4.6 and P < 0.001), and mir361-3p (fold change: 5.1 and P < 0.001)were downregulated compared to healthy individuals and mir-424 did not show any significant change between HCC patients and controls. Conclusion: Our results indicated that mir-33a, mir-203b, and mir-361-3p may significantly contribute to tumor pathogenesis in HCC and have potential to be used as a noninvasive biomarker for cancer therapy.Key words: Mir-33a, mir-203b, mir361-3p, mir-424, HCC, qRT-PCR
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- 1. Astarcı HM, Özyalvaçlı G, Sertçelik A. The diagnostic value of pCEA, mCEA, AFP and CK19 in differentiating primary and metastatic carcinoma of liver. Abant Medical Journal 2016; 5 (1): 39-46. doi: 10.5505/abantmedj.2016.94557
- 2. Llovet JM, Zucman-Rossi J, Pikarsky E, Sangro B, Schwartz M et al. Hepatocellular carcinoma. Nature Reviews Disease Primers 2016; 2. doi: 10.1038/nrdp.2016.18
- 3. Hamid AS, Tesfamariam IG, Zhang Y, Zhang ZG. Aflatoxin B1-induced hepatocellular carcinoma in developing countries: geographical distribution, mechanism of action and prevention. Oncology Letters 2013; 5 (4): 1087-92. doi: 10.3892/ol.2013.1169
- 4. Guzel E, Okyay TM, Yalçınkaya B, Karacaoglu S, Gocmen M et al. Tumor suppressor and oncogenic role of long non-coding rnas in cancer. Northern Clinics of İstanbul 2020; 7 (1): 81-86. doi:10.14744/nci.2019.46873
- 5. Yu L, Ding G, He C, Sun L, Jiang Y et al. MicroRNA-424 is down-regulated in hepatocellular carcinoma and suppresses cell migration and invasion through c-Myb. PLoS One 2014; 9 (3): e91661. doi: 10.1371/journal.pone.0091661
- 6. Jin Y, Wong YS, Goh BKP, Chan CY, Cheow PC et al. Circulating microRNAs as potential diagnostic and prognostic biomarkers in hepatocellular carcinoma. Scientific Reports 2019; 9 (1): 10464. doi: 10.1038/s41598-019-46872-8
- 7. Wang Y, Lee ATC, Ma JZI, Wang J, Ren J et al. Profiling microRNA expression in hepatocellular carcinoma reveals microRNA-224 up-regulation and apoptosis inhibitor-5 as a microRNA-224-specific target. Journal of Biological Chemistry 2008; 283 (19): 13205-13215. doi: 10.1074/jbc.m707629200
- 8. Xie RT, Cong XL, Zhong XM, Luo P, Yang HQ et al. MicroRNA33a downregulation is associated with tumorigenesis and poor prognosis in patients with hepatocellular carcinoma. Oncology Letters 2018; 1 (4): 4571-4577. doi: 10.3892/ol.2018.7892
- 9. Deng B, Wang B, Fang J, Zhu X, Cao Z et al. MiRNA-203 suppresses cell proliferation, migration and invasion in colorectal cancer via targeting of EIF5A2. Scientific Reports 2016; 6: 1-11. doi: 10.1038/srep28301
- 10. Xu X, Tao Y, Shan L, Chen R, Jiang H et al. The role of micrornas in hepatocellular carcinoma. Journal of Cancer 2018; 9 (19): 3557-3569. doi: 10.7150/jca.26350
- 11. Chen W, Wang J, Liu S, Wang S, Cheng Y et al. MicroRNA361-3p suppresses tumor cell proliferation and metastasis by directly targeting SH2B1 in NSCLC. Journal of Experimental and Clinical Cancer Research 2016; 35 (1): 1-16. doi: 10.1186/ s13046-016-0357-4
- 12. Supic G, Zeljic K, Rankov AD, Kozomara R, Nikolic A et al. miR-183 and miR-21 expression as biomarkers of progression and survival in tongue carcinoma patients. Clinical Oral Investigations 2018; 22 (1): 401-409. doi: 10.1007/s00784-017- 2126-y
- 13. Suer I, Guzel E, Karatas OF, Creighton CJ, Ittmann M et al. MicroRNAs s prognostic markers in prostate cancer. The Prostate 2019; 79 (3): 265-271. doi: 10.1002/pros.23731
- 14. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001; 25 (4): 402-408. doi: 10.1006/ meth.2001.1262
- 15. Eng J. ROC analysis: web-based calculator for ROC curves, Vol. 2010. Baltimore, MD, USA: Johns Hopkins University, 2006.
- 16. Sohel MH. Extracellular/circulating micrornas: release mechanisms, functions and challenges. Achievements in the Life Sciences 2016; 10 (2): 175-186. doi: 10.1016/j. als.2016.11.007
- 17. Kostopoulou F, Malizos KN, Papathanasiou I, Tsezou A. MicroRNA-33a regulates cholesterol synthesis and cholesterol efflux-related genes in osteoarthritic chondrocytes. Arthritis Research & Therapy 2015; 17 (1): 42. doi: 10.1186/s13075-015- 0556-y
- 18. Zhang C, Zhang Y, Ding W, Lin Y, Huang Z et al. MiR-33a suppresses breast cancer cell proliferation and metastasis by targeting ADAM9 and ROS1. Protein & Cell 2015; 6 (12): 881- 889. doi: 10.1007/s13238-015-0223-8
- 19. Boll K, Reiche K, Kasack K, Morbt N, Kretzschmar AK et al. MiR-130a, miR-203 and miR-205 jointly repress key oncogenic pathways and are downregulated in prostate carcinoma. Oncogene 2013; 32 (3): 277-285. doi: 10.1038/onc.2012.55
- 20. Iorio M V, Visone R, Di Leva G, Donati V, Petrocca F et al. MicroRNA signatures in human ovarian cancer. Cancer Research 2007; 67 (18): 8699-8707. doi: 10.1158/0008-5472. CAN-07-1936
- 21. Hou XW, Sun X, Yu Y, Zhao HM, Yang ZJ et al. miR-361-5p suppresses lung cancer cell lines progression by targeting FOXM1. Neoplasma 2017; 64 (4): 526-534. doi: 10.4149/ neo_2017_406
- 22. Murphy SJ, Lusardi TA, Phillips JI, Saugstad JA. Sex differences in microRNA expression during development in rat cortex. Neurochemistry International. 2014; 77: 24-32. doi: 10.1016/j. neuint.2014.06.007
| APA | Yalçınkaya B, Guzel Tanoglu E, TASTEKIN D, PENÇE S (2021). Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma. , 638 - 643. 10.3906/sag-2004-214 |
| Chicago | Yalçınkaya Burhanettin,Guzel Tanoglu Esra,TASTEKIN DIDEM,PENÇE SADRETTIN Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma. (2021): 638 - 643. 10.3906/sag-2004-214 |
| MLA | Yalçınkaya Burhanettin,Guzel Tanoglu Esra,TASTEKIN DIDEM,PENÇE SADRETTIN Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma. , 2021, ss.638 - 643. 10.3906/sag-2004-214 |
| AMA | Yalçınkaya B,Guzel Tanoglu E,TASTEKIN D,PENÇE S Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma. . 2021; 638 - 643. 10.3906/sag-2004-214 |
| Vancouver | Yalçınkaya B,Guzel Tanoglu E,TASTEKIN D,PENÇE S Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma. . 2021; 638 - 643. 10.3906/sag-2004-214 |
| IEEE | Yalçınkaya B,Guzel Tanoglu E,TASTEKIN D,PENÇE S "Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma." , ss.638 - 643, 2021. 10.3906/sag-2004-214 |
| ISNAD | Yalçınkaya, Burhanettin vd. "Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma". (2021), 638-643. https://doi.org/10.3906/sag-2004-214 |
| APA | Yalçınkaya B, Guzel Tanoglu E, TASTEKIN D, PENÇE S (2021). Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma. Turkish Journal of Medical Sciences, 51(2), 638 - 643. 10.3906/sag-2004-214 |
| Chicago | Yalçınkaya Burhanettin,Guzel Tanoglu Esra,TASTEKIN DIDEM,PENÇE SADRETTIN Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma. Turkish Journal of Medical Sciences 51, no.2 (2021): 638 - 643. 10.3906/sag-2004-214 |
| MLA | Yalçınkaya Burhanettin,Guzel Tanoglu Esra,TASTEKIN DIDEM,PENÇE SADRETTIN Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma. Turkish Journal of Medical Sciences, vol.51, no.2, 2021, ss.638 - 643. 10.3906/sag-2004-214 |
| AMA | Yalçınkaya B,Guzel Tanoglu E,TASTEKIN D,PENÇE S Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma. Turkish Journal of Medical Sciences. 2021; 51(2): 638 - 643. 10.3906/sag-2004-214 |
| Vancouver | Yalçınkaya B,Guzel Tanoglu E,TASTEKIN D,PENÇE S Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma. Turkish Journal of Medical Sciences. 2021; 51(2): 638 - 643. 10.3906/sag-2004-214 |
| IEEE | Yalçınkaya B,Guzel Tanoglu E,TASTEKIN D,PENÇE S "Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma." Turkish Journal of Medical Sciences, 51, ss.638 - 643, 2021. 10.3906/sag-2004-214 |
| ISNAD | Yalçınkaya, Burhanettin vd. "Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma". Turkish Journal of Medical Sciences 51/2 (2021), 638-643. https://doi.org/10.3906/sag-2004-214 |
