Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka

kirikci, koray; MERCAN, Levent; CAM, Mehmet Akif

doi:10.3906/vet-2009-40

Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka

Koray KIRIKÇI, (Kırşehir Ahi Evran Üniversitesi, Ziraat Fakültesi, Hayvan Bilimleri Bölümü, Kırşehir, Türkiye)

Mehmet Akif ÇAM, (Ondokuz Mayıs Üniversitesi, Ziraat Fakültesi, Hayvan Bilimleri Bölümü, Samsun, Türkiye)

Levent MERCAN (Ondokuz Mayıs Üniversitesi, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü, Samsun, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

9 0

Yıl: 2021 Cilt: 45 Sayı: 1 Sayfa Aralığı: 191 - 197 Metin Dili: İngilizce DOI: 10.3906/vet-2009-40 İndeks Tarihi: 28-01-2022

Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka

Öz:

Abstract: GDF9 is an essential gene for normal follicular development and prolificacy in sheep. The aim of this study was to determine the genetic polymorphism and G1 mutation of GDF9 gene exon 1 in 100 Karayaka ewes, belonging to four subpopulations in the Black Sea Region provinces of Turkey. Based on DNA sequence results, two alleles (A and G) and two genotypes (GG and GA) were determined with the restriction fragment length polymorphism analysis of PCR-amplified fragments (PCR-RFLP) method. The frequencies of mutant (A) and wild-type (G) alleles were detected as 0.10 and 0.90. The frequencies of GG and GA genotypes were calculated as 0.79 and 0.21, respectively. Twenty-one percent of the studied animals were heterozygous GA genotype. The values for observed (Ho) and expected (He) heterozygosity for GDF9-exon 1 were estimated as 0.210 and 0.189, respectively. DNA sequence results revealed that Karayaka breed had the mutation G1 (c.260G>A). In conclusion, this mutation might be used as a potential marker to improve litter size rate in Karayaka sheep as evidenced in other sheep breeds.Key words: Karayaka sheep, litter size, GDF9 gene exon 1, PCR-RFLP analysis, Hhal restriction enzyme

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APA	kirikci k, CAM M, MERCAN L (2021). Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka. , 191 - 197. 10.3906/vet-2009-40
Chicago	kirikci koray,CAM Mehmet Akif,MERCAN Levent Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka. (2021): 191 - 197. 10.3906/vet-2009-40
MLA	kirikci koray,CAM Mehmet Akif,MERCAN Levent Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka. , 2021, ss.191 - 197. 10.3906/vet-2009-40
AMA	kirikci k,CAM M,MERCAN L Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka. . 2021; 191 - 197. 10.3906/vet-2009-40
Vancouver	kirikci k,CAM M,MERCAN L Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka. . 2021; 191 - 197. 10.3906/vet-2009-40
IEEE	kirikci k,CAM M,MERCAN L "Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka." , ss.191 - 197, 2021. 10.3906/vet-2009-40
ISNAD	kirikci, koray vd. "Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka". (2021), 191-197. https://doi.org/10.3906/vet-2009-40

APA	kirikci k, CAM M, MERCAN L (2021). Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka. Turkish Journal of Veterinary and Animal Sciences, 45(1), 191 - 197. 10.3906/vet-2009-40
Chicago	kirikci koray,CAM Mehmet Akif,MERCAN Levent Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka. Turkish Journal of Veterinary and Animal Sciences 45, no.1 (2021): 191 - 197. 10.3906/vet-2009-40
MLA	kirikci koray,CAM Mehmet Akif,MERCAN Levent Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka. Turkish Journal of Veterinary and Animal Sciences, vol.45, no.1, 2021, ss.191 - 197. 10.3906/vet-2009-40
AMA	kirikci k,CAM M,MERCAN L Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka. Turkish Journal of Veterinary and Animal Sciences. 2021; 45(1): 191 - 197. 10.3906/vet-2009-40
Vancouver	kirikci k,CAM M,MERCAN L Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka. Turkish Journal of Veterinary and Animal Sciences. 2021; 45(1): 191 - 197. 10.3906/vet-2009-40
IEEE	kirikci k,CAM M,MERCAN L "Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka." Turkish Journal of Veterinary and Animal Sciences, 45, ss.191 - 197, 2021. 10.3906/vet-2009-40
ISNAD	kirikci, koray vd. "Investigation of G1 (c.260G>A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka". Turkish Journal of Veterinary and Animal Sciences 45/1 (2021), 191-197. https://doi.org/10.3906/vet-2009-40