Introduction: The aim of this study was to investigate the relationships between homophobia and gender roles of university students. Methodology: This descriptive study was completed with 1048 students studying at a state university. The data was collected using the Information Form, the Gender Roles Attitudes Scale and the Attitudes Towards Homosexuals Scale. The study data were analyzed in computer environment using SPSS 21 package program. While evaluating the data, the conformity of the variables to the normal distribution was analyzed with the Shapiro Wilks test. Findings: The average age of the students was 20,39±2,23, and 43.9% male. The average score of the attitues towards homosexuals scale of the students was 86,71±24,88, and 49,04% of them got a score above the average on the scale. The average gender roles attitudes scale score of the students is 66,49±22,33. It was found that there were statistically significant positive correlations (r=,310-,651; p=,000) between gender roles attitudes scale total and sub-dimension scores and attitues towards homosexuuals scale scores. Descriptive statistical analyzes of sociodemographic characteristics and scale score distribution were analyzed using the Student's t test if the two independent variables were compatible with the normal distribution, and with the Mann Whitney U test when they were not. Relationships between scale scores were analyzed by Pearson correlation analysis. Significance was accepted at p<0.05 for all analyzes. Conclusion: University students' participating in the research attitudes towards gender roles are more traditional and approximately half of them have homophobic attitudes towards homosexuals. Gender roles attitues and homophobia are associated with university students and as the scores from the gender roles scale increase, the scores from the homophobia scale also increase.