Yıl: 2022 Cilt: 25 Sayı: 1 Sayfa Aralığı: 19 - 24 Metin Dili: İngilizce DOI: 10.18016/ksutarimdoga.vi.884279 İndeks Tarihi: 31-08-2022

Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery

Öz:
The increase in the frequency of drug resistance in bacterial infections has led to the research of new antibacterial agents targeting new mechanisms. Many of the functions of NAD+-dependent DNA ligase have made it a remarkable target for antibacterial drug discovery. Escherichia coli (E. coli) NAD+-dependent DNA ligase is presented as a potential target due to its unique substrate specificity compared to the ATP-dependent human DNA ligase. In this study, it was aimed to produce and purify the E. coli NAD + dependent DNA ligase enzyme, which is frequently used in antibacterial drug discovery. The E. coli DNA ligase gene sequence was cloned into pTOLT vector system. E. coli DNA ligase enzyme was purified after the production in E. coli BL21 (DE3) pLysE cells. It was clearly demonstrated by the activity test that the DNA ligase enzyme produced in this study can ligate the DNA fragments. As a result, it was revealed that the effect of candidate inhibitors can be studied simply on the enzyme.
Anahtar Kelime:

Antibakteriyel İlaç Keşfi İçin Bir Hedef Olarak E. Coli NAD+-Bağımlı DNA Ligazın Rekombinant Üretimi

Öz:
Bakteriyel enfeksiyonlarda ilaç direnci frekansındaki artış, yeni mekanizmaları hedefleyen yeni antibakteriyel ajanların araştırılmasına yol açmıştır. NAD+-bağımlı DNA ligazın fonksiyonlarının bir çoğu, onu antibakteriyel ilaç keşfi için dikkate değer bir hedef haline getirmiştir. Escherichia coli (E. coli) NAD+ bağımlı DNA ligazı, ATP bağımlı insan DNA ligazı ile karşılaştrıldığında, benzersiz substrat özgüllüğü nedeniyle potansiyel bir hedef olarak görülmektedir. Bu çalışmada antibakteriyel ilaç keşiflerinde sıklıkla kullanılan E. coli NAD+ bağımlı DNA ligaz enziminin, yüksek miktarda ve saflıkta üretilmesi amaçlamıştır. E. coli DNA ligaz gen dizisi, pTOLT vektör sistemine klonlanmıştır. E. coli DNA ligaz enzimi, E. coli BL21 (DE3) pLysE hücrelerinde üretildikten sonra saflaştırılmıştır. Bu çalışmada üretilen DNA ligaz enziminin DNA fragmanlarının ligasyonunu sağlayabildiği, aktivite testi ile açıkça gösterilmiştir. Sonuç olarak, bu enzim üzerinde aday inhibitörlerin etkisinin basitçe incelenebileceği ortaya koyulmuştur.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Kaplan O, IMAMOGLU R, GOKCE I (2022). Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery. , 19 - 24. 10.18016/ksutarimdoga.vi.884279
Chicago Kaplan Ozlem,IMAMOGLU RIZVAN,GOKCE Isa Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery. (2022): 19 - 24. 10.18016/ksutarimdoga.vi.884279
MLA Kaplan Ozlem,IMAMOGLU RIZVAN,GOKCE Isa Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery. , 2022, ss.19 - 24. 10.18016/ksutarimdoga.vi.884279
AMA Kaplan O,IMAMOGLU R,GOKCE I Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery. . 2022; 19 - 24. 10.18016/ksutarimdoga.vi.884279
Vancouver Kaplan O,IMAMOGLU R,GOKCE I Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery. . 2022; 19 - 24. 10.18016/ksutarimdoga.vi.884279
IEEE Kaplan O,IMAMOGLU R,GOKCE I "Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery." , ss.19 - 24, 2022. 10.18016/ksutarimdoga.vi.884279
ISNAD Kaplan, Ozlem vd. "Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery". (2022), 19-24. https://doi.org/10.18016/ksutarimdoga.vi.884279
APA Kaplan O, IMAMOGLU R, GOKCE I (2022). Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(1), 19 - 24. 10.18016/ksutarimdoga.vi.884279
Chicago Kaplan Ozlem,IMAMOGLU RIZVAN,GOKCE Isa Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 25, no.1 (2022): 19 - 24. 10.18016/ksutarimdoga.vi.884279
MLA Kaplan Ozlem,IMAMOGLU RIZVAN,GOKCE Isa Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, vol.25, no.1, 2022, ss.19 - 24. 10.18016/ksutarimdoga.vi.884279
AMA Kaplan O,IMAMOGLU R,GOKCE I Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2022; 25(1): 19 - 24. 10.18016/ksutarimdoga.vi.884279
Vancouver Kaplan O,IMAMOGLU R,GOKCE I Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2022; 25(1): 19 - 24. 10.18016/ksutarimdoga.vi.884279
IEEE Kaplan O,IMAMOGLU R,GOKCE I "Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery." Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25, ss.19 - 24, 2022. 10.18016/ksutarimdoga.vi.884279
ISNAD Kaplan, Ozlem vd. "Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial Drug Discovery". Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 25/1 (2022), 19-24. https://doi.org/10.18016/ksutarimdoga.vi.884279