Objective: The prevalence of Health care-related infections is higher in the ICU than in other units of the hospital because of frequent central venous catheters, urinary catheters, mechanical ventilation and long-term hospitalizations. This study was undertaken to describe the impact of various risk factors and the antibiotic resistance of breeding microorganisms in the Health care related infections epidemiology acquired in the internal tertiary ICU. Materials and Methods: A retrospective study was carried out on prospectively collected data of 62 patients who applied internally at tertiary ICU between January 2014 and December 2017. The Health care related infections were diagnosed according to the Centers for Disease and Control (CDC) definitions. All patients' data were obtained from hospital information management system and infection control committee patient follow-up forms. Results: 36 (58.1%) of the cases were male and 26 (41.9%) were female. The causative agent of infection is primarily A. baumannii, K. pneumoniae and E. coli. The most common infection was found as urinary tract infection with 34%. In our study, all A. baumannii strains were carbapenem resistant. The ESBL positivity rate in K. pneumoniae was found to be 62.5%, while all E. coli strains were found to be positive for EBSL. Methicillin resistance was present in all of the coagulase (-) staphylococci. Conclusion: It is also evident that a larger scale of work with a larger number of patients are needed, although each hospital considers that such studies are important in terms of knowing the infection frequency, factors and resistance profile in their unit.