Objective: This study was conducted for psychometric assessment of the nursing and midwifery students’ attitudes towards sexual health (SA-SH) scale. Material and Methods: Methodological design was used in this research. The entire population of the study consisted of a total of 333 nursing and midwifery (188 and 145) students enrolled at the 3rd and 4th years of the Faculty of Health Sciences at Kahramanmaraş Sütçü İmam University in Turkey. No sample selection method was used in the study, and the entire population was aimed to be reached. The study was completed with a total of 280 students (174 nursing, 106 midwifery), and 84% of the entire population was reached. The sample size in the study was sufficient for factor analysis. The data were collected by using the descriptive questionnaire form and the SASH scale. Results: The SA-SH scale consists of 3 factors and 15 items. The Cronbach’s alpha coefficient was calculated as 0.87. The KaiserMeyer-Olkin value was 0.88, and the Bartlett’s test statistic was found as chi-square (χ2 )=2546.409, p=0.000. The factor analysis revealed that the items were collected under 3 factors with eigenvalues of higher than 1, and these factors explained 67.1% of the total variance. Conclusion: With the validity and reliability study that was conducted, it was determined that the scale is suitable for nursing and midwifery students and a valid and reliable measurement instrument in assessing the attitudes of students towards sexual health. It is recommended to apply the scale with different groups of students and a larger sample.