Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and  D-Dimer Levels in Calf Diarrhea in Neonatal Period

AKTAS, MUSTAFA SINAN; Yanar, Kerim Emre; Eroğlu, Muhammed Sertaç; EREN, EMRE

doi:10.9775/kvfd.2022.27662

Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period

Mustafa Sinan AKTAŞ, (Atatürk Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Erzurum, Türkiye)

Muhammed Sertaç EROĞLU, (Atatürk Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Erzurum, Türkiye)

Kerim Emre YANAR, (Atatürk Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Erzurum, Türkiye)

Emre EREN (Atatürk Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Erzurum, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

9 1

Yıl: 2022 Cilt: 28 Sayı: 4 Sayfa Aralığı: 507 - 514 Metin Dili: İngilizce DOI: 10.9775/kvfd.2022.27662 İndeks Tarihi: 14-09-2022

Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period

Öz:

In this study, it was aimed to evaluate the relationship between the clinical course of the disease and hematological data, serum 25-hydroxyvitamin D₃ (25 (OH) D₃), iron (Fe), free iron-binding capacity (UIBC), and D-dimer levels in calves with diarrhea in the neonatal period. Within the scope of the study, 10 healthy calves (group-I) and 30 diarrheal calves in the neonatal period of diff erent races, ages and genders were used. Calves with diarrhea were divided into mild (group-II, n=10), moderate (group-III, n=10) and severe (group-IV, n=10) groups. Blood samples were taken from calves in all groups at once. Hematological analyzes were performed using a veterinary-specific hematology analyzer device. In serum samples, 25 (OH) D3, Fe and UIBC levels were determined with an autoanalyzer, and D-dimer levels were determined with an automatic immunoassay analyzer. In the hematological analysis, an increase was observed in the number of LYMs (lymphocytes) in group-II (5.04±1.3) and III (5.2±3.3) compared to group-I (4.47±1.2), and a decrease was observed in group IV (2.76±0.9) (P<0.05). Fe levels in group-II (59±56), group III (56±52) and group IV (72±63) were found to be decreased compared to group-I (131±66) (P<0.05). It was determined that the 25 (OH) D3 level of group IV (13.4±8.5) was higher than that of group-I (6.12±2.73) (P<0.05). D-dimer levels of group-III (1.15±1.13) and group-IV (0.96±0.88) were found to be higher than group-I (0.10±1.46) (P<0.05).

Anahtar Kelime:

Neonatal Dönem İshalli Buzağılarda 25 (OH) D₃, Demir, Demir Bağlama Kapasitesi ve D-Dimer Düzeylerinin Belirlenmesi

Öz:

Bu çalışmada neonatal dönem ishalli buzağılarda hastalığın klinik seyri ile hematolojik veriler, serum 25-hidroksivitamin D₃ (25 (OH) D₃), demir (Fe), serbest demir bağlama kapasitesi (UIBC) ve D-dimer düzeyleri arasındaki ilişkinin değerlendirilmesi amaçlandı. Çalışma kapsamında neonatal dönemde değişik ırk, yaş ve cinsiyetteki 10 sağlıklı (grup-I) ve 30 ishalli buzağı kullanıldı. İshalli buzağılar, hafif (grup-II, n=10) orta (grup-III, n=10) ve şiddetli (grup-IV, n=10) olmak üzere gruplara ayrıldı. Tüm gruplardaki buzağılardan bir kereye mahsus olmak üzere kan örnekleri alındı. Hematolojik analizler veteriner spesifik hemogram cihazı kullanılarak yapıldı. Serum örneklerinde 25 (OH) D₃, Fe ve UIBC düzeyleri otoanalizör cihazı ile, D-dimer düzeyleri ise otomatik immunoassay analizör cihazı ile belirlendi. Hematolojik analizlerde LYM (lenfosit) sayılarında grup-II (5.04±1.3) ve III’de (5.2±3.3) grup-I’e göre (4.47±1.2) artış, grup IV’de ise (2.76±0.9) bir azalış şekillendiği belirlendi (P<0.05). Grup-II (59±56), grup III (56±52) ve grup IV (72±63) Fe düzeylerinin grup-I’e göre (131±66) azaldığı belirlendi (P<0.05). Grup-IV’ün 25 (OH) D₃ düzeyinin (13.4±8.5) grup-I’e göre (6.12±2.73) yüksek olduğu belirlendi (P<0.05). Grup-III (1.15±1.13) ve grup-IV’ün (0.96±0.88) D-dimer düzeylerinin grup-I’e göre (0.10±1.46) yüksek olduğu belirlendi (P<0.05).

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Erkilic EE, Merhan O, Kirmizigul A, Ogun M, Akyuz E, Citil M: Salivary and serum levels of serum amyloid a, haptoglobin, ceruloplasmin and albumin in neonatal calves with diarrhoea. Kafkas Univ Vet Fak Derg, 25 (4): 583-586, 2019. DOI: 10.9775/kvfd.2018.21424
2. Ozcan U, Sezener MG, Sayılkan BU, Ergüden VE, Küllük E, Yaman Ş: A new aspect in neonatal calf diarrhea: Presence of Escherichia coli CS31A at unexpected ratio. Kafkas Univ Vet Fak Derg, 27 (1): 133-134, 2021. DOI: 10.9775/kvfd.2020.25280
3. Keleş I, Ekinci G, Tüfekçi E, Çitil M, Güneş V, Aslan O, Onmaz AC, Karaca Bekdik İ, Varol K, Deniz O: Etiological and predisposing factors in calves with neonatal diarrhea: A clinical study in 270 case series. Kafkas Univ Vet Fak Derg, 28 (3): 315-326, 2022. DOI: 10.9775/kvfd.2021.26981
4. Akyüz E, Sezer M, Kuru M, Naseri A: Changes in hematology, some clinical biochemical parameters and mineral levels in neonatal calves with sepsis due to diarrhea. Van Vet J, 33 (1): 26-30, 2022. DOI: 10.36483/ vanvetj.1066050
5. Özaydın I, Erkılıç EE, Kılıç E, Yayla S, Baran V: Yenidoğan buzağılarda sıvı-elektrolit sağaltımı. In, Baran V (Ed): Ruminantlarda Neonatal Cerrahi. 105-110, Türkiye Klinikleri, Ankara 2019.
6. Kozat S, Tuncay İ: Siirt yöresindeki yenidoğan ishalli buzağılarda Rotavirus, Coronavirus, Cryptosporidium spp., Escherichia coli K 99 ve Giardia lamblia etkenlerinin prevalansı. Van Vet J, 29 (1): 17-22, 2018.
7. Peinado VI, Celdrán JF, Palomeque J: Basic hematological values in some wild ruminants in captivity. Comp Biochem Physiol A Mol Integr Physiol, 124 (2): 199-203, 1999. DOI: 10.1016/S1095-6433(99)00110-5
8. Karatoy Erdem B, Akbas H: D vitamini ve metabolomik. Turkish J Biochem, 16 (2): 127-194, 2018.
9. Haque UJ, Bathon JM, Giles JT: Association of vitamin D with cardiometabolic risk factors in rheumatoid arthritis. Arthritis Care Res, 64 (10): 1497-1504, 2012. DOI: 10.1002/acr.21715
10. Ahmed AM: Association of vitamin D status with acute respiratory infection and diarrhoea in children less than two years of age in an urban slum of Bangladesh. PhD Thesis. The University of Queensland, 2016.
11. Cherayil BJ: The role of iron in the immune response to bacterial infection. Immunol Res, 50 (1): 1-9, 2011. DOI: 10.1007/S12026-010-8199-1
12. Ayoglu H, Sezer U, Akin M, Okyay D, Ayoglu F, Can M, Kucukosman G, Piskin O, Aydin B, Cimencan M, Gur A, Turan I: Selenium, copper, zinc, iron levels and mortality in patients with sepsis and systemic inflammatory response syndrome in Western Black Sea Region, Turkey. J Pak Med Assoc, 66 (4): 447-452, 2016.
13. Elmasoğlu O: Akut ishalli buzağılarda serum demir, bakır ve çinko konsantrasyonlarının değerlendirilmesi. MSc Thesis. Adnan Menderes University Institute of Health Sciences, 2008.
14. Bick RL: Disseminated intravascular coagulation: Objective clinical and laboratory diagnosis, treatment, and assessment of therapeutic response. Semin Thromb Hemost, 22 (1): 69-88, 1996. DOI: 10.1055/S-2007-998993
15. Grünberg W: Diarrhea in neonatal ruminants. https://www. merckvetmanual.com/digestive-system/intestinal-diseases-in-ruminants/ diarrhea-in-neonatal-ruminants; Accessed: 12.03.2022.
16. Sayers RG, Kennedy A, Krump L, Sayers GP, Kennedy E: An observational study using blood gas analysis to assess neonatal calf diarrhea and subsequent recovery with a european commission-compliant oral electrolyte solution. Int J Dairy Sci, 99 (6): 4647-4655, 2016. DOI: 10.3168/ jds.2015-10600
17. Walker PG, Constable PD, Morin DE, Drackley JK, Foreman JH, Thurmon JC: A reliable, practical, and economical protocol for inducing diarrhea and severe dehydration in the neonatal calf. Can J Vet Res, 62 (3): 205, 1998.
18. Fecteau G, Pare J, Van Metre DC, Smith BP, Holmberg CA, Guterbock W, Jang S: Use of a clinical sepsis score for predicting bacteremia in neonatal dairy calves on a calf rearing farm. Can Vet J, 38 (2): 101, 1997.
19. Kozat S, Özkan C, Okman EN: Evaluation of homocysteine levels in neonatal calves with diarrhea. Kafkas Univ Vet Fak Derg, 27 (3): 271-277, 2021. DOI: 10.9775/kvfd.2020.24894
20. Cho YI, Yoon KJ: An overview of calf diarrhea infectious etiology, diagnosis, and intervention. J Vet Sci, 15 (1): 1-17, 2014. DOI: 10.4142/JVS.2014.15.1.1
21. Atcalı T, Yıldız R: Neonatal buzağı ishallerinde farklı etiyolojik faktörlerin hemogram parametreleri üzerine etkisi. MAKÜ Sağlık Bil Enst Derg, 8 (3): 119-127, 2020. DOI: 10.24998/maeusabed.823819
22. Ranjan Barua S, Md Rakib T, Das S, Masuduzzaman M, Alamgir Hossain M, Chowdhury S: Hematological and serological changes in neonatal diarrheic calves infected with bovine rotavirus. Multidiscip Adv Vet Sci, 2 (3): 356-366, 2018.
23. Reis JP, von Mühlen D, Miller ER, Michos ED, Appel LJ: Vitamin D status and cardiometabolic risk factors in the united states adolescent population. Pediatrics, 124 (3): 371-379, 2009. DOI: 10.1542/peds.2009-0213
24. Gudmundsson GH, Bergman P, Andersson J, Raqib R, Agerberth B: Battle and balance at mucosal surfaces the story of Shigella and antimicrobial peptides. Biochem Biophys Res Commun, 396 (1): 116-119, 2010. DOI: 10.1016/j.bbrc.2010.03.081
25. Kong J, Zhang Z, Musch MW, Ning G, Sun J, Hart J, Bissonnette M, Li YC: Novel role of the vitamin D receptor in maintaining the integrity of the intestinal mucosal barrier. Am J Physiol Gastrointest Liver Physiol, 294 (1): G208–G216, 2008. DOI: 10.1152/AJPGI.00398.2007
26. Çamkerten G, Erdoğan H, Ural DA, Çamkerten İ, Erdoğan S, Ural K: Giardia duodenalis ile doğal ile enfekte kuzularda serum 25 (OH) D3 seviyeleri. Kocatepe Vet J, 12 (1): 71-74, 2019. DOI: 10.30607/kvj.492805
27. Erdoğan S, Alıç Ural D, Erdoğan H, Ayan A, Ural K, Özalp T, Günal İ: Evaluation of serum 25-hydroxy vitamin D3 levels in goat kids naturally infected with Giardia duodenalis. J Adv VetBio Sci Tech, 5 (2): 43-47, 2020. DOI: 10.31797/vetbio.696080
28. Özalp T, Erdoğan H: Evaluation of 25 (OH) D3 concentrations in neonatal calves with diarrhea. Kocatepe Vet J, 12 (3): 268-276, 2019. DOI: 10.30607/kvj.532206
29. Adeeb F, Khan M, Li X, Stack A, Devlin J, Fraser AD: High vitamin D levels may downregulate inflammation in patients with Behçet’s Disease. Int J Inflam, 2017:8608716, 2017. DOI: 10.1155/2017/8608716
30. Grodzki K, Lechowski R, Archiwum MLP: The biochemical profile of calves’ liver in the course of diarrhea during the first 10 days of life. Pol Arch Weter, 31 (3-4): 49-63, 1991.
31. Prosser DE, Jones G: Enzymes involved in the activation and inactivation of vitamin D. Trends Biochem Sci, 29 (12): 664-673, 2004. DOI: 10.1016/j. tibs.2004.10.005
32. Smith GW: Treatment of calf diarrhea: Oral fluid therapy. Vet Clin North Am Food Anim Pract, 25 (1): 55-72, 2009. DOI: 10.1016/j.cvfa.2008.10.006
33. Cappellini M, Cohen A, Eleftheriou A, Piga A, Porter J: Guidelines for the clinical Management of Thalassaemia. https://www.ncbi.nlm.nih.gov/ books/NBK173968/; Accessed: 13.03.2022.
34. Yoshimi O: Anemia in newborn calves and effect of supplemental iron agents. J Livest Med, 53 (2): 105-111, 2006.
35. Baydar E, Dabak M: Serum iron as an indicator of acute inflammation in cattle. Int J Dairy Sci, 97 (1): 222-228, 2014. DOI: 10.3168/jds.2013-6939
36. Aydoğdu U, Coşkun A, Yıldız R, Güzelbekteş H, Şen İ: Sistemik yangısal cevap sendromlu buzağılarda hematolojik parametreler ve serum demir düzeyindeki değişimler. Eurasian J Vet Sci, 34 (1): 56-59, 2018. DOI: 10.15312/EurasianJVetSci.2018.180
37. Piercy DWT: Acute phase responses to experimental salmonellosis in calves and colibacillosis in chickens: serum iron and caeruloplasmin. J Comp Pathol, 89 (3): 309-319, 1979. DOI: 10.1016/0021-9975(79)90020-3
38. Basoglu A, Sen I, Sevinc M, Simsek A: Serum concentrations of tumor necrosis factor a in neonatal calves with presumed septicemia. J Vet Intern Med, 18 (2): 238-241, 2004. DOI: 10.1111/j.1939-1676.2004.tb00167.x
39. Blombäck B, Hessel B, Hogg D, Therkildsen L: A two-step fibrinogen fibrin transition in blood coagulation. Nature, 275 (5680): 501-505, 1978. DOI: 10.1038/275501a0
40. Özatlı D: D-dimer laboratuvardan güncel pratiğe. 35. Ulusal Hematoloji Kongresi. 7-10 Ekim, Antalya, 2009.
41. Mammen EF: Oral contraceptive pills and hormonal replacement therapy and thromboembolic disease. Hematol Oncol Clin North Am, 14 (5): 1045- 1059, 2000. DOI: 10.1016/S0889-8588(05)70170-2
42. Cıngı CÇ, Kabu M, Erdoğan H, Haydardedeoğlu AE, Ural K: Determination of d-dimer levels in calves with cryptosporidiosis. Kocatepe Vet J, 12 (4): 396-399, 2019. DOI: 10.30607/kvj.599089
43. Sobiech P, Rekawek W, Ali M, Targonski R, Zarczynska K, Snarska A, Stopyra A: Changes in blood acid-base balance parameters and coagulation profile during diarrhea in calves. Pol J Vet Sci, 16 (3): 543-549, 2013. DOI: 10.2478/pjvs-2013-0075
44. Naseri A, Şen İ, Turgut K, Guzelbektes H: Evaluation of coagulation abnormalities and cardiac biomarkers in calves with naturally occurring severe sepsis or septic shock. Van Vet J, 32 (1): 28-32, 2021. DOI: 10.36483/ VANVETJ.855449

APA	AKTAS M, Eroğlu M, Yanar K, EREN E (2022). Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period. , 507 - 514. 10.9775/kvfd.2022.27662
Chicago	AKTAS MUSTAFA SINAN,Eroğlu Muhammed Sertaç,Yanar Kerim Emre,EREN EMRE Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period. (2022): 507 - 514. 10.9775/kvfd.2022.27662
MLA	AKTAS MUSTAFA SINAN,Eroğlu Muhammed Sertaç,Yanar Kerim Emre,EREN EMRE Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period. , 2022, ss.507 - 514. 10.9775/kvfd.2022.27662
AMA	AKTAS M,Eroğlu M,Yanar K,EREN E Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period. . 2022; 507 - 514. 10.9775/kvfd.2022.27662
Vancouver	AKTAS M,Eroğlu M,Yanar K,EREN E Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period. . 2022; 507 - 514. 10.9775/kvfd.2022.27662
IEEE	AKTAS M,Eroğlu M,Yanar K,EREN E "Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period." , ss.507 - 514, 2022. 10.9775/kvfd.2022.27662
ISNAD	AKTAS, MUSTAFA SINAN vd. "Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period". (2022), 507-514. https://doi.org/10.9775/kvfd.2022.27662

APA	AKTAS M, Eroğlu M, Yanar K, EREN E (2022). Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(4), 507 - 514. 10.9775/kvfd.2022.27662
Chicago	AKTAS MUSTAFA SINAN,Eroğlu Muhammed Sertaç,Yanar Kerim Emre,EREN EMRE Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28, no.4 (2022): 507 - 514. 10.9775/kvfd.2022.27662
MLA	AKTAS MUSTAFA SINAN,Eroğlu Muhammed Sertaç,Yanar Kerim Emre,EREN EMRE Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.28, no.4, 2022, ss.507 - 514. 10.9775/kvfd.2022.27662
AMA	AKTAS M,Eroğlu M,Yanar K,EREN E Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(4): 507 - 514. 10.9775/kvfd.2022.27662
Vancouver	AKTAS M,Eroğlu M,Yanar K,EREN E Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(4): 507 - 514. 10.9775/kvfd.2022.27662
IEEE	AKTAS M,Eroğlu M,Yanar K,EREN E "Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, ss.507 - 514, 2022. 10.9775/kvfd.2022.27662
ISNAD	AKTAS, MUSTAFA SINAN vd. "Determination of 25 (OH) D3, Iron, Free Iron Binding Capacity and D-Dimer Levels in Calf Diarrhea in Neonatal Period". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28/4 (2022), 507-514. https://doi.org/10.9775/kvfd.2022.27662