In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves

Ceren, Rümeysa; Aydın, Enes; Renda, Gülin; Barut, Burak

doi:10.55730/1300-0527.3552

In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves

Gülin RENDA, (Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Trabzon, Türkiye)

Burak BARUT, (Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Trabzon, Türkiye)

Rümeysa CEREN, (Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Trabzon, Türkiye)

Enes AYDIN (Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Trabzon, Türkiye)

Turkish Journal of Chemistry

3 0

Yıl: 2023 Cilt: 47 Sayı: 2 Sayfa Aralığı: 465 - 475 Metin Dili: İngilizce DOI: 10.55730/1300-0527.3552 İndeks Tarihi: 12-06-2023

In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves

Öz:

Turkey is the world’s leading producer of figs, a typical Mediterranean fruit. The fig, Ficus carica L. (Moraceae), has been widely cultivated since ancient times due to the nutritional value of its fruits. It was aimed to investigate the phytochemical characterization and biological properties of F. carica leaf extracts in order to determine their potential for use in the treatment of various diseases. F. carica leaves were extracted in 70% methanol at 40 °C under reflux. To obtain extracts of different polarities, the crude extract was fractionated with n-hexane, dichloromethane, and n-butanol. Phenolic content was determined using liquid chromatography–high resolution mass spectrometry (LC–HRMS). 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and antityrosinase activities of all extracts were investigated using spectrophotometric methods. Furthermore, the DNA-damage protective properties of extracts were investigated using electrophoretic methods. The n-butanol extract was found to have the highest total phenolic content, with 72.58 ± 4.52 mg GAE/g dry weight. According to LC–HRMS analysis, rutin (40.13 g/kg) was the most abundant compound in the n-butanol extract. The n-butanol extract, which was found to have the highest tyrosinase inhibitory effects among the extracts, demonstrated radical scavenging activity of 37.01 ± 1.15% and 82.57 ± 0.88% at 80 and 200 μg/mL, respectively. The n-butanol extract had the highest protective effects against Fenton’s reagent, UV radiation, and singlet oxygen. Given these findings, it is possible to argue that F. carica leaves can be evaluated for developing products that could be used to treat various diseases.

Anahtar Kelime: F. carica LC-HRMS plasmid pBR322 DNA rutin tyrosinase inhibitory

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Renda G, Barut B, Ceren R, Aydın E (2023). In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves. , 465 - 475. 10.55730/1300-0527.3552
Chicago	Renda Gülin,Barut Burak,Ceren Rümeysa,Aydın Enes In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves. (2023): 465 - 475. 10.55730/1300-0527.3552
MLA	Renda Gülin,Barut Burak,Ceren Rümeysa,Aydın Enes In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves. , 2023, ss.465 - 475. 10.55730/1300-0527.3552
AMA	Renda G,Barut B,Ceren R,Aydın E In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves. . 2023; 465 - 475. 10.55730/1300-0527.3552
Vancouver	Renda G,Barut B,Ceren R,Aydın E In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves. . 2023; 465 - 475. 10.55730/1300-0527.3552
IEEE	Renda G,Barut B,Ceren R,Aydın E "In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves." , ss.465 - 475, 2023. 10.55730/1300-0527.3552
ISNAD	Renda, Gülin vd. "In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves". (2023), 465-475. https://doi.org/10.55730/1300-0527.3552

APA	Renda G, Barut B, Ceren R, Aydın E (2023). In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves. Turkish Journal of Chemistry, 47(2), 465 - 475. 10.55730/1300-0527.3552
Chicago	Renda Gülin,Barut Burak,Ceren Rümeysa,Aydın Enes In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves. Turkish Journal of Chemistry 47, no.2 (2023): 465 - 475. 10.55730/1300-0527.3552
MLA	Renda Gülin,Barut Burak,Ceren Rümeysa,Aydın Enes In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves. Turkish Journal of Chemistry, vol.47, no.2, 2023, ss.465 - 475. 10.55730/1300-0527.3552
AMA	Renda G,Barut B,Ceren R,Aydın E In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves. Turkish Journal of Chemistry. 2023; 47(2): 465 - 475. 10.55730/1300-0527.3552
Vancouver	Renda G,Barut B,Ceren R,Aydın E In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves. Turkish Journal of Chemistry. 2023; 47(2): 465 - 475. 10.55730/1300-0527.3552
IEEE	Renda G,Barut B,Ceren R,Aydın E "In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves." Turkish Journal of Chemistry, 47, ss.465 - 475, 2023. 10.55730/1300-0527.3552
ISNAD	Renda, Gülin vd. "In vitro tyrosinase inhibitory, DNA interaction studies, and LC–HRMS analysis of Ficus carica leaves". Turkish Journal of Chemistry 47/2 (2023), 465-475. https://doi.org/10.55730/1300-0527.3552