Objective: While motivation is a very important issue for every institution, it may be said that it is of vital importance at hospitals, where healthcare services are provided, for patients, their relatives and people who provide the services. In this research, it was aimed to examine the stress caused by problems that final year students of nursing school encounter during clinical practice and its relationship with motivation. Methods: This research, for which a descriptive and relational research design has been used, was conducted in the 2015-2016 school year among 167 nursing interns enrolled in a faculty of nursing and volunteered to participate in the research. The students were evaluated during practice. Necessary ethics approval was obtained before conducting the research. The "Introductory Information Form", the "Scale for Sources and Problems of Motivation" and the "Perceived Stress Scale" were used to collect data. These data were evaluated using numbers, percentage distributions and the tests measuring the significance of the difference between independent groups. Findings: The mean age of the students participated in the survey was 25.37 ± 1.71 and 79.1% of them were females. %78.5 of the students stated that they encountered the cases, which decrease the motivation. The motivation scores among those, who perceived the presence of academic staff at the clinic for the short periods of time as a negative situation (U = 1191.00, p <0.05), and the stress scores of those who perceived it as a positive situation (U = 970.50, P<0.05) were found to be significantly higher. The motivation scores of the students who had the "fear of making mistakes at the clinic" (U = 1206.50, P <0.05) and those who did not have problems in "preparing the care plan" (U=1430.00, P<0.05); the stress scores of those "who wanted to be independent during the administration of treatment" (U = 508.50, P <0.05) and those who did not want to "work on the tasks they did not like " (U=827.50, p>0,05) were found to be statistically significantly higher. Besides, there was a negative correlation at the significance level of 0.01 (p = 0.01) between the total item scores on the "Perceived Stress Scale" and on the "Scale for Sources and Problems of Motivation" (r = -0,211). Conclusion: The results of the research showed that there is a relationship between the sources of stress that the students encounter during the clinical practice and their motivation and that the stressors faced by the students during the clinical practice have an effect on their motivation levels.