Objective: This study was planned to evaluate the nurses’ professional self-concept, personality traits, and occupational perceptions and to examine the relationship between means of scales. Method: It was planned as a descriptive and correlational research. The research sample was made of 185 nurses working in two private hospitals and a medical center in Istanbul. Data were obtained using the Perception of Nursing Profession Scale, Ten-Item Personality Scale, and the Professional Self-Concept Scale in Nurses. Results: The average age of nurses was 25.32±5.02, 42.2% had a bachelor’s degree. The mean of experience of sample was 5.21±4.70 years. Perception of Nursing Profession Scale scores showed that professional self-concept was quite positive. scores of the statements of the Ten-Item Personality Scale were “I consider myself as reliable self-disciplined”, “I consider myself as sympathetic, warm-hearted” and “I consider myself as extroverted, enthusiastic” was higher than the means of other items. A statistically significant positive relationship was found between the scores of Extroversion subscale and Professional Satisfaction subscale (p=0.006). There were not significant relationships between the scores of Perception of Nursing Profession Scale and personality scale (p>0.05). The analysis revealed a significant relationship between the scores of Professional Self-Concept Scale in Nurses and Perception of Nursing Profession Scale (p<0.01). Conclusion: The positive relationship between nurses’ occupational perceptions and professional self-perceptions revealed the need for strategies to improve the nurses’ perceptions about the profession. Approaches are needed to be adopted for improving the occupational perceptions and professional self-perception by the personality traits.