Objective: This study was conducted to examine the relationship between cancer knowledge burden and healthy lifestyle behaviors of female workers. Methods: The research was conducted with a descriptive and relationship seeking female workers working in the shift system in the city center of Karaman. The number of female workers in the factories has been kept confidential as per the corporate policy. For this reason, the total number of female workers could not be reached. This research was completed with 380 women workers. The data were collected using the Questionnaire Form, Cancer Information Overload Scale and Healthy Lifestyle Behaviors II Scale prepared by the researcher. Pearson correlation analysis, t test and Mann Whitney U test were used in data analysis. Independent variables that had an effect on women's cancer knowledge overload and healthy lifestyle behavior scores in the primary analyzes were evaluated by multiple regression analysis. Results: %70.6 of female workers are 31 years old and over, %51.3 of them have received education at the literate/primary level. %95.3 of the participants heard about cancer screening tests, while %57.9 heard from healthcare professionals, %42.1 heard from the press/broadcast. Cancer Information Overload Scale mean score of female workers is 19.32±5.38, Healthy Lifestyle Behaviors II Scale mean score is 119.14±22.29. It was determined that there was a weak and positive significant relationship between the Cancer Information Overload Scale scores of female workers and the nutrition, spiritual development and interpersonal relations sub-dimension scores of the Healthy Lifestyle Behaviors II Scale (p<0.05). Conclusion: It was concluded that as the cancer knowledge load of female workers increased, healthy lifestyle behaviors such as nutrition, spiritual development and interpersonal relations also increased.