Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease

KARATAŞ, Özhan; NUHOĞLU, HİLMİ; YILDIZ, Ayfer; BEYTUT, ENVER; DAĞ, SERPİL; Mendil, Ali Sefa; Karakurt, Emin

Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease

Emin KARAKURT, (Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kars, Türkiye)

Özhan KARATAŞ, (Cumhuriyet Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Sivas, Türkiye)

Serpil DAG, (Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kars, Türkiye)

Enver BEYTUT, (Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kars, Türkiye)

Ali Sefa MENDİL, (Erciyes Üniversitesi, Sağlık Bilimleri Enstitüsü, Kayseri, Türkiye)

Hilmi NUHOĞLU, (Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kars, Türkiye)

Ayfer YILDIZ (Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kars, Türkiye)

Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi

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Yıl: 2021 Cilt: 35 Sayı: 2 Sayfa Aralığı: 109 - 113 Metin Dili: İngilizce İndeks Tarihi: 24-01-2022

Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease

Öz:

The aim of this study was to investigate Reactive Oxygen Species (ROS)-induced lipidperoxidation, protein and DNA damage with oxidative stress markers such as 4-hydroxy-2- nonenal (4-HNE), dityrosine (DT) and 8-hydroxy-2-deoxyguanosine (8-OHdG) in lambs withWhite Muscle Disease (WMD). The material of this study consisted of tissue samples taken from20 sheep with WMD and 6 healthy control groups brought to Pathology Department for routinehistopathological diagnosis between 2012 and 2019. In macroscopic examinations, chalky-whitelarge necrotic areas on the epicardial and especially on the endocardial surfaces of the right andleft ventricular walls were observed. In microscopic examinations, it was observed that thedegenerated muscle fibers in the heart were homogeneously pink in color, lost their cytoplasmiccitration, swelled and their nuclei were pyknotic. Calcifications in necrotic and degenerated areaswere detected. All white muscle disease cases were immune positive for 4-HNE, DT and 8-OHdGexpressions in heart tissues. A significant difference (P<0.05) was detected between the groups interms of immunoreactivity to 4-HNE, DT and 8-OHdG in immunohistochemical andimmunofluorescence staining findings. In conclusion, our study showed that formation of ROS thatis important in pathogenesis of WMD causes not only lipid peroxidation, but also proteinmodification and DNA damage.

Anahtar Kelime:

Beyaz Kas Hastalıklı Kuzularda 4-Hidroksi-2-Nonenal, Ditirozin and 8-Hidroksi-2- Deoksiguanozin Ekspresyonlarının Değerlendirilmesi

Öz:

Bu çalışmada, beyaz kas hastalıklı kuzularda 4-hidroksi-2-nonenal (4-HNE), ditirozin (DT) ve 8- hidroksi-2-deoksiguanozin (8-OHdG) gibi oksidatif stres belirteçleri ile Reaktif Oksijen Türleri(ROT) kaynaklı lipid peroksidasyonu, protein ve DNA hasarının araştırılması amaçlanmıştır. Buçalışmanın materyalini 2012-2019 yılları arasında rutin histopatolojik tanı için Patoloji AnabilimDalı’na getirilen beyaz kas hastalıklı 20 koyundan alınan doku örnekleri ve 6 sağlıklı kontrol grubuoluşturdu. 4-HNE, DT ve 8-OHdG ekspresyonları immünohistokimyasal ve immünofloresanyöntemlerle değerlendirildi. Makroskopik incelemelerde epikardiyal ve özellikle endokardiyalyüzeylerde, sağ ve sol ventrikül duvarlarında tebeşirimsi beyaz büyük nekrotik alanlar gözlemlendi.Mikroskobik incelemelerde kalpteki dejenere olmuş kas liflerinin homojen pembe renkte olduğunu,sitoplazmik sitrifikasyonunu kaybettiğini, şiştiği ve çekirdeklerinin piknotik olduğu gözlemlendi.Nekrotik ve dejenere alanlarda kalsifikasyonlar tespit edildi. Tüm beyaz kas hastalığı vakaları kalpdokularında 4-HNE, DT ve 8-OHdG ekspresyonu yönünden immün pozitifti. İmmünohistokimyasalve immünofloresan boyama sonuçlarında 4-HNE, DT ve 8-OHdG'ye karşı immünreaktiviteaçısından gruplar arasında anlamlı farklılık (P<0.05) tespit edildi. Sonuç olarak çalışmamız beyazkas hastalığının patogenezinde önemli bir faktör olan ROS oluşumunun hem lipidperoksidasyonuna, hem protein modifikasyonuna hem de DNA hasarına neden olduğunugöstermektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Karakurt E, KARATAŞ Ö, DAĞ S, BEYTUT E, Mendil A, NUHOĞLU H, YILDIZ A (2021). Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease. , 109 - 113.
Chicago	Karakurt Emin,KARATAŞ Özhan,DAĞ SERPİL,BEYTUT ENVER,Mendil Ali Sefa,NUHOĞLU HİLMİ,YILDIZ Ayfer Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease. (2021): 109 - 113.
MLA	Karakurt Emin,KARATAŞ Özhan,DAĞ SERPİL,BEYTUT ENVER,Mendil Ali Sefa,NUHOĞLU HİLMİ,YILDIZ Ayfer Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease. , 2021, ss.109 - 113.
AMA	Karakurt E,KARATAŞ Ö,DAĞ S,BEYTUT E,Mendil A,NUHOĞLU H,YILDIZ A Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease. . 2021; 109 - 113.
Vancouver	Karakurt E,KARATAŞ Ö,DAĞ S,BEYTUT E,Mendil A,NUHOĞLU H,YILDIZ A Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease. . 2021; 109 - 113.
IEEE	Karakurt E,KARATAŞ Ö,DAĞ S,BEYTUT E,Mendil A,NUHOĞLU H,YILDIZ A "Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease." , ss.109 - 113, 2021.
ISNAD	Karakurt, Emin vd. "Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease". (2021), 109-113.

APA	Karakurt E, KARATAŞ Ö, DAĞ S, BEYTUT E, Mendil A, NUHOĞLU H, YILDIZ A (2021). Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 35(2), 109 - 113.
Chicago	Karakurt Emin,KARATAŞ Özhan,DAĞ SERPİL,BEYTUT ENVER,Mendil Ali Sefa,NUHOĞLU HİLMİ,YILDIZ Ayfer Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi 35, no.2 (2021): 109 - 113.
MLA	Karakurt Emin,KARATAŞ Özhan,DAĞ SERPİL,BEYTUT ENVER,Mendil Ali Sefa,NUHOĞLU HİLMİ,YILDIZ Ayfer Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, vol.35, no.2, 2021, ss.109 - 113.
AMA	Karakurt E,KARATAŞ Ö,DAĞ S,BEYTUT E,Mendil A,NUHOĞLU H,YILDIZ A Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi. 2021; 35(2): 109 - 113.
Vancouver	Karakurt E,KARATAŞ Ö,DAĞ S,BEYTUT E,Mendil A,NUHOĞLU H,YILDIZ A Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi. 2021; 35(2): 109 - 113.
IEEE	Karakurt E,KARATAŞ Ö,DAĞ S,BEYTUT E,Mendil A,NUHOĞLU H,YILDIZ A "Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease." Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 35, ss.109 - 113, 2021.
ISNAD	Karakurt, Emin vd. "Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease". Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi 35/2 (2021), 109-113.