Yıl: 2022 Cilt: 38 Sayı: 2 Sayfa Aralığı: 83 - 89 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2022.368

Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection

Öz:
Aim: Bovine adenovirus (BAdV) is one of the viral agents that causes respiratory illness in cattle, along with numerous other viral agents. Although the prevalence of BAdV has been determined by serological studies conducted in our country, there are limited data on the detection of BAdVs in respiratory system samples, and there is no study on their molecular characterization. The aim of this study was to detect and characterize BAdVs of the Mastadenovirus genus. Materials and Methods: In this study, a total of 64 nasal swabs and one lung sample from 65 cattle of different ages with respiratory system disease symptoms were used. After the extraction of viral DNA, they were tested by PCR using primers targeting the hexon gene region and the expected size of amplicons were sequenced. Results: According to the PCR results, three samples were found positive and the positivity rate was detected as 4.6%. Out of positive samples, two were able to be sequenced and one clustered in the BAdV-2 serotype while the other was grouped in the BAdV-3 serotype. Conclusion: In this study, it was revealed that BAdVs are contributing agents in respiratory system infection in our country. Also, this is the first study reporting the molecular characterization of BAdV-2 and BAdV-3 in calves with respiratory system infection in Turkey.
Anahtar Kelime:

Konular:
Fen > Veterinerlik > Veterinerlik
Fen > Veterinerlik

Solunum sistemi enfeksiyonu olan buzağılarda mastadenovirusların tespiti ve moleküler karakterizasyonu

Öz:
Amaç: Bovine adenoviruslar (BAdV), birçok viral etkenle birlikte sığırlarda solunum sistemi hastalığına neden olan patojenlerden biri olarak kabul edilmektedir. Ülkemizde BAdV prevalansı, yapılan serolojik çalışmalarla tespit edilmiş olmasına rağmen, solunum sistemi örneklerinde BAdV'nin saptanmasına ilişkin veriler sınırlıdır ve moleküler karakterizasyonları ile ilgili herhangi bir çalışma bulunmamaktadır. Bu çalışmada Mastadenovirus genusunda bulunan BAdV’ların tespiti ve moleküler karakterizasyonu amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada, solunum sistemi hastalığı semptomları olan farklı yaştaki 65 sığırdan alınan toplam 64 burun sürüntüsü ve bir akciğer örneği kullanıldı. Viral DNA'nın ekstraksiyonunu takiben, örnekler hekzon gen bölgesini hedefleyen primerler kullanılarak PCR ile test edildi ve beklenen büyüklükteki amplikonların dizin bilgisi elde edildi. Bulgular: PCR sonucunda üç örnek pozitif bulunmuş ve pozitiflik oranı %4,6 olarak tespit edilmiştir. Dizin bilgileri elde edilebilen iki örnekten birinin BAdV-2 serotipinde, diğerinin ise BAdV-3 serotipinde yer aldığı saptanmıştır. Öneri: Bu çalışmada BAdV'lerin ülkemizde solunum sistemi enfeksiyonuna katkıda bulunan ajanlar olduğu ortaya koyulmuştur. Ayrıca bu, Türkiye'de solunum sistemi enfeksiyonu olan buzağılarda BAdV-2 ve BAdV-3'ün moleküler karakterizasyonunu bildiren ilk çalışmadır
Anahtar Kelime:

Konular:
Fen > Veterinerlik > Veterinerlik
Fen > Veterinerlik
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APA KARAYEL HACIOĞLU İ, ALKAN F, YELKEN S (2022). Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. Eurasian Journal of Veterinary Sciences, 38(2), 83 - 89. 10.15312/EurasianJVetSci.2022.368
Chicago KARAYEL HACIOĞLU İlke,ALKAN Feray,YELKEN Selda DURAN Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. Eurasian Journal of Veterinary Sciences 38, no.2 (2022): 83 - 89. 10.15312/EurasianJVetSci.2022.368
MLA KARAYEL HACIOĞLU İlke,ALKAN Feray,YELKEN Selda DURAN Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. Eurasian Journal of Veterinary Sciences, vol.38, no.2, 2022, ss.83 - 89. 10.15312/EurasianJVetSci.2022.368
AMA KARAYEL HACIOĞLU İ,ALKAN F,YELKEN S Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. Eurasian Journal of Veterinary Sciences. 2022; 38(2): 83 - 89. 10.15312/EurasianJVetSci.2022.368
Vancouver KARAYEL HACIOĞLU İ,ALKAN F,YELKEN S Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. Eurasian Journal of Veterinary Sciences. 2022; 38(2): 83 - 89. 10.15312/EurasianJVetSci.2022.368
IEEE KARAYEL HACIOĞLU İ,ALKAN F,YELKEN S "Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection." Eurasian Journal of Veterinary Sciences, 38, ss.83 - 89, 2022. 10.15312/EurasianJVetSci.2022.368